Active Plants, Animals & Ecology Genetics & Molecular Biology

COEXIST-Maximising data from fragmented bone to understand the coexistence of late Neanderthals and early Homo sapiens in central and southeast Europe

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AI plain-English summary

Seventy to ninety percent of ancient bone fragments found at archaeological sites are routinely thrown away because researchers cannot tell whether they came from a human, a cow, or a bear. A new method called ZooMS identifies these fragments by reading the collagen signatures in each piece, and this project will apply it to 14,000 bone samples from central and southeast Europe, dated to between 55,000 and 45,000 years old. The goal is to reconstruct how late Neanderthals and early *Homo sapiens* lived alongside each other—what they ate, how they butchered animals, and whether differences in subsistence drove Neanderthal disappearance. Any human remains identified through ZooMS will also be analysed with proteomics and ancient DNA to detect episodes of interbreeding. This is fundamental science: it addresses a long-standing gap in understanding the behavioural dynamics of population interactions and replacements in our deep past. If successful, the project will provide the first large-scale, multidisciplinary chronology of Neanderthal-*Homo sapiens* coexistence in the region, shifting current hypotheses about how one human species replaced another.

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While recent studies of ancient DNA have been revolutionary in demonstrating we interbred with Neanderthals, the chronological and geographic extent of these interactions, as well as any behavioural variations, remain poorly understood. In COEXIST, I will apply the latest technical advances from archaeological science, proteomics and genetics to reconstruct Neanderthal-Homo sapiens coexistence, which is essential to understand how we became the only human species in the world. Small bone fragments make up 70-90% of archaeological bone collections but are often excluded from analyses, as it is not known what type of animal or human they belonged to. The method of Zooarchaeology by Mass Spectrometry (ZooMS) can now identify these fragments through variations in their collagen, allowing insights into human behaviour, as well as discoveries of new human remains. In this project, I will apply ZooMS to 14,000 bone samples, dated to 55,000-45,000 years old, from central and southeast Europe, a region with recent evidence for an early presence of Homo sapiens. First, with these ZooMS identifications, I will reconstruct how late Neanderthals and early Homo sapiens used a site, what animals they ate, and how they selected, acquired, and butchered them. I can then test if differences in subsistence played a role in Neanderthal disappearance. Second, any human remains identified through ZooMS will be analysed using the latest proteomic and DNA methods to assess their genetic histories, including episodes of interbreeding. Finally, applying the latest advances in sample selection for radiocarbon dating, I will establish a new chronology for Neanderthal-Homo sapiens coexistence in the study region. Through its ambitious, large-scale and multidisciplinary approach COEXIST will have a significant impact on current hypotheses on Neanderthal-Homo sapiens coexistence and shift current views on the behavioural dynamics of population interactions and replacements in our deep past.

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Researchers

Karen Ruebens (Principal Investigator)

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