A grass leaf is a living map, and its boundaries are drawn by cells deciding where one domain ends and another begins. This project will reveal the mechanism that draws those lines. How a shapeless cluster of cells becomes a complex organ like a leaf is one of the great unsolved puzzles in biology. The grass leaf is an ideal model because its base-to-tip structure contains distinct domains with clear functions—such as the blade, sheath, and ligule—that are critical for photosynthesis and growth. Current tools have been too crude to watch this process in real time. The researcher has now combined live imaging, single-cell RNA sequencing, spatial protein analysis, and computational modelling to track domain formation as it happens. If successful, this work will uncover a fundamental patterning mechanism shared across plant organs and species. Understanding how shape is controlled at the cellular level could eventually allow precise engineering of crops for traits like drought tolerance or yield. This is fundamental science—there is no immediate practical application—but similar work on plant development has previously underpinned the Green Revolution and modern crop breeding.
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"from so simple a beginning endless forms most beautiful and most wonderful have been and are being evolved" (Darwin). This is not only true for diversity in species, but also during development. From amorphous groups of cells complex tissues and organs form, combining to make an organism able to interact with its environment. How organ shape is defined, whether there is a common underlying mechanism, and how this has been adapted to generate diverse shapes, are core unanswered questions. DynaLines will reveal a fundamental patterning mechanism that defines plant organ shape and how it has been altered over evolution to generate morphological innovation. A key step in organ development is the delineation of groups of cells with unique identities. Plant organ shape arises due to these domains growing at different rates and influences all aspects of plant life. Despite the importance of domain patterning, the components and how they are regulated in the dynamic context of a developing plant organ is poorly understood. The base-to-tip of the grass leaf is an excellent model as it has distinct domains with agronomically important functions. To date studies have been hampered by a lack of tools, inaccessibility of the developing leaves and genetic redundancy. Single-cell RNAseq and spatial protein analysis combined with my recent advances in live imaging, computational modelling and transgenic tools in grasses now makes this possible. Using these tools, I will reveal the mechanism that delineates domains in the grass leaf, identifying components that define domains and placing them in a spatial and temporal context for the first time. Comparing different organs and species will reveal the conserved mechanism and identify key changes behind shape diversity. My novel approach will be a step-change in our understanding of plant development and evolution, and will uncover a mechanism important for key agronomic traits, paving the way for precision engineering of crops.
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